Journal: Polymers
Article Title: Magnesium Hydroxide as a Versatile Nanofiller for 3D-Printed PLA Bone Scaffolds
doi: 10.3390/polym16020198
Figure Lengend Snippet: ALP staining images of BMSCs after culturing for 7 and 14 days: PLA ( a , d ), PLA/5Mg(OH) 2 ( b , e ), PLA/20Mg(OH) 2 ( c , f ). Notes: More cells with a higher degree of staining are observed in PLA/5Mg(OH) 2 , but only a large amount of whisker-like substances are seen in PLA/20Mg(OH) 2 , indicating that the appropriate addition of Mg(OH) 2 is beneficial for enhancing the osteogenic differentiation ability of cells.
Article Snippet: The materials used in the experiments are mainly included: PLA (REVODE, Zhejiang Hisun Biomaterials Co., Ltd., Taizhou, China); Mg(OH) 2 nanopowders (~200 nm, as shown in ) (Beijing Deke Science and Technology Co., Ltd., Beijing, China); dichloromethane (CH 2 Cl 2 , ≥99.5%, Sichuan Xilong Science Co., Ltd., Chengdu, China); simulated body fluid (SBF) (R24167, 1.5×, Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China); rabbit bone marrow mesenchymal stem cells (BMSCs) (RBXMX-01001) and BMSC complete culture medium (RBXMX-80011) (Cyagen Biosciences, Inc., Guangzhou, China); DAPI staining solution (C1005), actin-tracker red-rhodamine (C2207S), BCIP/NBT alkaline phosphatase color development kit (C3206) (Shanghai Beyotime Biotechnology Co., Ltd., Shanghai, China); and phosphate buffered solution (Yida Technology Co., Ltd., Quanzhou, China).
Techniques: Staining, Whisker Assay